Antifibrotic effects of sulforaphane treatment on gingival elasticity reduces orthodontic relapse after rotational tooth movement in beagle dogs
김경님, 김주영, 차정열, 최성환, 김진, 조성원, 황충주,
소속 상세정보
김경님 ( Kim Kyong-Nim ) - Yonsei University College of Dentistry Department of Orthodontics
김주영 ( Kim Jue-Young ) - Yonsei University College of Dentistry Department of Oral Pathology
차정열 ( Cha Jung-Yul ) - Yonsei University College of Dentistry Department of Orthodontics
최성환 ( Choi Sung-Hwan ) - Yonsei University College of Dentistry Department of Orthodontics
김진 ( Kim Jin ) - Yonsei University College of Dentistry Department of Oral Pathology
조성원 ( Cho Sung-Won ) - Yonsei University College of Dentistry Department of Oral Biology
황충주 ( Hwang Chung-Ju ) - Yonsei University College of Dentistry Department of Orthodontics
Abstract
Objective: Increased gingival elasticity has been implicated as the cause of relapse following orthodontic rotational tooth movement and approaches to reduce relapse are limited. This study aimed to investigate the effects of sulforaphane (SFN), an inhibitor of osteoclastogenesis, on gene expression in gingival fibroblasts and relapse after rotational tooth movement in beagle dogs.
Methods: The lower lateral incisors of five beagle dogs were rotated. SFN or dimethylsulfoxide (DMSO) were injected into the supra-alveolar gingiva of the experimental and control group, respectively, and the effect of SFN on relapse tendency was evaluated. Changes in mRNA expression of extracellular matrix components associated with gingival elasticity in beagles were investigated by real-time polymerase chain reaction. Morphology and arrangement of collagen fibers were observed on Masson’s trichrome staining of buccal gingival tissues of experimental and control teeth.
Results: SFN reduced the amount and percentage of relapse of orthodontic rotation. It also decreased the gene expression of lysyl oxidase and increased the gene expression of matrix metalloproteinase (MMP) 1 and MMP 12, compared with DMSO control subjects. Histologically, collagen fiber bundles were arranged irregularly and were not well connected in the SFN-treated group, whereas the fibers extended in parallel and perpendicular directions toward the gingiva and alveolar bone in a more regular and well-ordered arrangement in the DMSO-treated group.
Conclusions: Our findings demonstrated that SFN treatment may be a promising pharmacologic approach to prevent orthodontic rotational relapse caused by increased gingival elasticity of rotated teeth in beagle dogs.
키워드
Rotation; Relapse; Gingival elasticity; Sulforaphane
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